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Overproduction of thermoalkalophilic lipase secreted by Bacillus atrophaeus FSHM2 using UV-induced mutagenesis and statistical optimization of medium components

Prep Biochem Biotechnol. 2019 Feb 3:1-8. doi: 10.1080/10826068.2019.1566148. [Epub ahead of print]Overproduction of thermoalkalophilic lipase secreted by Bacillus atrophaeus FSHM2 using UV-induced mutagenesis and statistical optimization of medium components.Ameri A1, Shakibaie M2,3, Soleimani-Kermani M4, Faramarzi MA5, Forootanfar H1,2.Author information1a Pharmaceutics Research Center, Institute of Neuropharmacology , Kerman University of Medical Sciences , Kerman , Iran.2b Department of Pharmaceutical Biotechnology, Faculty of Pharmacy , Kerman University of Medical Sciences , Kerman , Iran.3c Herbal and Traditional Medicines Research Center , Kerman University of Medical Sciences , Kerman , Iran.4d The Student Research Committee, Faculty of Pharmacy , Kerman University of Medical Sciences , Kerman , Iran.5e Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Biotechnology Research Center , Tehran University of Medical Sciences , Tehran , Iran.AbstractMicrobial enzymes of extremophilic origin serve as a vital source of stable industrial enzymes. The present study focused on overproduction of a thermoalkalophilic lipase produced by Bacillus atrophaeus FSHM2 through UV-induced random mutagenesis (5-45 min exposure to UV light) and factorial experimental design augmented to response surface methodology. Firstly, a UV-induced mutant (designated as UV-45) was developed after the exposure of wild strain to UV irradiation for 45 min which was able to secrete 3484.8 U/L lipase. Afterward, Plackett-Burman experimental approach augmented to central composite design was employed to optimize medium components (olive oil, maltose, glucose, sucrose, yeast extract, tryptone, urea, (NH4)2SO4, NaCl, CaCl2, and ZnSO4) for lipase production by the UV-45 mutant strain. The maximum lipase production of 5505.3 U/L were predicted in medium containing 5% of olive oil, 0.69% of glucose, 0.69% of sucrose, 2.5% of maltose, yeast extract (0.7 g/L), urea (0.44 g/L), (NH4)2SO4 (2.44 g/L), tryptone (1.19 g/L), NaCl (1.61 g/L), CaCl2 (3.81 g/L), and ZnSO4 (1.42 g/L). A mean value of 5161.3 ± 83.3 U/L of lipolytic activity was acquired from real experiments. To sum up, the lipolytic activity of wild type strain (1720.4 U/L) increased by 3-fold after UV-induced mutagenesis and medium components optimization (5161.3 U/L).KEYWORDS: ; Lipase; Medium optimization; UV-induced mutagenesisPMID: 30712452 DOI: 10.1080/10826068.2019.1566148 ShareLinkOut - more resourcesFull Text SourcesTaylor & Francis

تولید آنزیم لیپاز thermoalkalophilic لیپاز توسط atrophaeus Bacillus FSHM۲ با استفاده از جهش زایی ناشی از اشعه فرابنفش و بهینه‌سازی آماری اجزای محیط کشت

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